Our Research & Scientific Contributions

Pioneering studies in plant tissue culture, biodiversity conservation, and sustainable agricultural biotechnology by Dr. Ashok Kumar Khandel & collaborators.

7Publications
3Journals
2023–24Recent Work
Open AccessMost Papers
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Plant Tissue Culture & In Vitro Propagation

4 Papers
Frontiers in Horticulture 2024 Open Access
Mosoh D.A., Khandel A.K., Verma S.K., Vendrame W.A.

This study establishes an efficient protocol for rapid in vitro propagation of the endangered glory lily (Gloriosa superba L.) through callus-mediated organogenesis using non-dormant corm explants. Treatment with 2.0 mg L⁻¹ BAP, 0.5 mg L⁻¹ NAA, 5 mg L⁻¹ CH, and 20% CW yielded a shooting response rate of 83.33%. The findings contribute to conserving this critically important medicinal plant species.

In Vitro Cell. Dev. Biol. – Plant 2023 Springer Nature
Mosoh D.A., Khandel A.K., Verma S.K., Vendrame W.A.

Evaluated the effect of plant growth regulators and sterilizing agents on in vitro propagation and micro-tuberization of endangered Gloriosa superba L. Treatment with 10% H₂O₂ for 5 min exhibited 82.14% viability and no contamination. Full-strength MS medium with 2.0 mg L⁻¹ BAP and 0.5 mg L⁻¹ NAA displayed 90.63% bud break with significant shoot elongation. 1009 accesses, 25 citations.

Tropical Plants 2024 Open Access
Mosoh D.A., Khandel A.K., Verma S.K., Vendrame W.A.

Sterilizing seeds with 0.15% mercuric chloride for 8 minutes effectively eliminated contamination, ensuring 100% seedling survival. MS medium with 1.5 mg·L⁻¹ GA₃ and 1.5 mg·L⁻¹ BAP along with 4% sucrose achieved the highest in vitro seed germination. The first systematic protocol addressing dual dormancy in this critically endangered tropical medicinal plant.

Tropical Plants 2024 Open Access
Mosoh D.A., Khandel A.K., Verma S.K., Vendrame W.A.

First report on a multi-plant approach for in vitro plant regeneration through direct organogenesis in Gloriosa superba L. The highest shoot induction rate of 91.7% was achieved from non-dormant corm explants with BAP and adenine sulfate. This multi-explant in vitro propagation approach is recommended for wider conservation efforts using plant tissue culture.

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Biodiversity & Conservation Science

2 Papers
Frontiers in Conservation Science 2024 Open Access
Mosoh D.A., Prakash O., Khandel A.K., Vendrame W.A.

A comprehensive literature review (1952–2024) examining the interplay between climate change and biodiversity conservation as two of the most urgent challenges of the 21st century. Analyzes IPCC projections for 2030, 2050, and 2100 using conservative values across six official Assessment Reports between 1990 and 2022.

Plant Cell, Tissue & Organ Culture 2024 Springer Nature
Mosoh D.A., Khandel A.K., Verma S.K., Vendrame W.A.

Investigates the phytochemical profile and alkaloid enhancement in callus cultures of Gloriosa superba using plant growth regulators and abiotic stress elicitors. The study advances understanding of secondary metabolite production in this medicinally valuable endangered species, supporting both conservation and pharmaceutical research goals.

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Phytochemistry & Medicinal Plant Research

1 Paper
Frontiers in Horticulture 2024 Open Access
Mosoh D.A., Khandel A.K., Verma S.K., Vendrame W.A.

Comprehensive assessment of alkaloid profiles — particularly colchicine and colchicoside — in callus cultures derived from Gloriosa superba L. The research evaluates biotechnological strategies to enhance yield of these high-value pharmaceutical alkaloids without harvesting from wild populations, directly supporting conservation and sustainable production goals.

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